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KMID : 0382619870070020705
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Hanyang Journal of Medicine
1987 Volume.7 No. 2 p.705 ~ p.719
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Effect of Gentamicin on Activities of Alkaline Phosphatase, Acid Phosphatase and Adenosine Triphosphatase in Liver of Mice.
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Abstract
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Gentamicin isolated from Micromonospora purpurea has been used as an antibiotic against a wide range of bacteria, including Staphylococci, Streptococci, Pneuynococ-ci, Escherichia coli, Pseudomonas and Proteus species. It is well known that gentamicin inhibits the protein synthesis of bacteria by interfering in the first step of the syn-thesis that occurs at the ribosome (initiation). Like all other aminoglycosides, gen-tamicin (at high does) has toxic effects on the kidney and the inner ear.
In this experiment, the author studied the effect of gentamicin on the mouse liver histochemically, observing the change in the activities of alkaline phosphatase, acid phosphatase and adenosine ´triphosphatase.
Healthy male mice (ICR strain) were used as experimental animals which were devided irito two groups; the control and gentamicin treated groiups. All experimental animal were given food and water ad libiturn.The animals´ of experimental group were administrated gentamicin, in a dose of 100 mg per kg of body weight by intramuscular injection. The experimental animals were sacrificed at 6, 12, 24 and 36 hours after administration of gentamicin, respectively. The liver specimens sectioned by cryostat were stained by Gomori method for studying changes in the activities of alkaline phosphatase, acid phosphatase and by Wachstein and Meisel method for adenosine triphosphatase, respectively.
The results obtained were as follows;
1. The activity of alkaline phosphatase exhibited strong positive reaction in the hepatic lobule of normal control group. As the time passed by, the activity of alkaline phosphatase was decreased in the hepatic lobule. Moderate positive activities of the enzyme were observed in the hepatic lobule of gentamicin treated group at 6 and 12 hours after administration. Weak positive reaction was shown at 24 hours after administration. Moderate positive reaction was seen in the hepatic lobule of gentamicin treated group at 36 hours after administration.
2. The activity of acid phosphatase exhibited weak positive reactions in the hepatic lobule of normal control group and the gentamicin treated group at 6 hours after administration. As the time passed by, the activities of enzyme were increased in the hepatic lobule. Moderate positive reaction of the enzyme was observed in the hepatic lobule of gentamicin treated group at 12 hours after administration. Str-ong positive reaction of the enzyme was observed in hepatic lobule of gentamicintreated group at 24 hours after administration. Weak positive reaction was shown in the hepatic lobule of gentamicin treated group at 36 hours after administration.
3. The activity of adenosine triphosphatase exhibited strong positive reaction inthe hepatic lobule of normal control group. Weak positive activities of enzymewere observed in the hepatic lobules of gentamicin treated group at 6, 12 and 24hours after administration. Moderate positive reaction was shown in the hepaticlobule of gentamicin treated group at 36 hours after administration.
Consequently, it is suggested that gentamicin damages the liver cell of mouse pro-bably by decreasing the activities of alkaline phosphatase and adenosine triphosphatase and by increasing the activity of acid phosphatase.
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